A real-time polymerase chain reaction (real-time PCR) is a laboratory technique of molecular biology based on the polymerase chain reaction(PCR). It monitors the amplification of a targeted DNA molecule during the PCR (i.e., in real time), not at its end, as in conventional PCR. Real-time PCR can be used quantitatively (quantitative real-time PCR) and semi-quantitatively (i.e., above/below a certain amount of DNA molecules) (semi-quantitative real-time PCR). Real-time PCR has become one of the indispensable detection means in the field of molecular biology with the advantages of high sensitivity, specificity, rapidity and simplicity.
However, the current fluorescence quantitative PCR in conventional laboratories have very limited application scenarios due to their large size and strong dependence on power supply. With the increasing demand for instant detection in the field, Unicorn Medical launched a portable fluorescence quantitative PCR — Q320, which has the advantages of being vehicle-accessible and easy to carry. It can be adapted to diverse and complex use environments.
More application scenarios of real-time PCR
The Q320 fluorescence quantitative PCR is simple and light, with a sample throughput of 2×16 wells. The use of the instrument is not limited by the scenarios. It can be applied in food and agriculture testing, consumer genomics, environmental microbiology, genetic research, forensic science, phylogenetics, commodity inspection, disaster/epidemic prevention and other instant detection, as well as Simple scientific research scenarios.
Product Features of Real-time PCR Q320
Real-time PCR is carried out in a thermal cycler with the capacity to illuminate each sample with a beam of light of at least one specified wavelength and detect the fluorescence emitted by the excited fluorophore. The thermal cycler is also able to rapidly heat and chill samples, thereby taking advantage of the physicochemical properties of the nucleic acids and DNA polymerase.
Excellent temperature control performance
Excellent temperature control is the key element to ensure successful experiments and accurate results. Q320 adopts imported semiconductors combined with efficient Peltier temperature control technology with a temperature control range of 4-100 °C to ensure the accuracy and uniformity while reaching a greater temperature ramp rate of up to 8 °C/s, effectively reducing experimental time as well as the generation of non-specific amplification.
Multi-channel detection capability
As a portable fluorescent quantitative PCR, Q320 has Multi-channel detection capability, which is compatible with some common fluorescent dyes on the market, such as FAM/SYBR Green I, VIC/HEX/JOE/TET, ROX, CY5etc. It can detect 4 target genes in the same reaction tube simultaneously. The Q320 has been validated to accept a minimum reaction volume of 15 μl, enabling more efficient use of precious samples while saving reagent costs.
New optical path design
The Q320 fluorescence quantitative PCR is equipped with a high-performance optical system that uses ultra-high brightness monochrome LEDs as the light source, which requires no preheating for power-on, which will save more energy and extend service life. The fluorescence acquisition device uses a high-sensitivity photoelectric detector with low background noise.
Software analysis function
Q320 fluorescence quantitative PCR analysis software supports common analysis applications, including qualitative analysis, absolute/relative quantification, melting curve, etc. According to different experimental purposes, the corresponding fluorescent PCR reagents can be selected to achieve many applications in the fields of qualitative/quantitative pathogen detection, drug efficacy evaluation, genetic disease screening, gene expression level research, food safety, genetically modified animal and plant testing, epidemic prevention and control, etc.
Unicorn Medical is a company integrated R&D, manufacturing, sales and service that provides complete and reliable digital medical and laboratory solutions in hundreds of countries and regions around the world. After decades of development. Unicorn has become a large scale laboratory equipment manufacturer and supplier all over the world.
Unicorn can provide different equipment plans according to different life science laboratories. The products involve ult freezers, laboratory refrigerators, high/low-speed centrifuge, biosafety cabinet, laminar flow clean bench, water, incubator, PCR instrument, low-temperature cold chain equipments and so on. Excellent R& D team, high-quality sales team and technical comprehensive, experienced after-sales service team, can provide users with quality products and satisfactory user experience.
