QuantaVue 4X Real-time PCR

The QuantaVue 4X series represents the pinnacle of Unicorn’s advancement in quantitative PCR technology. It’s a time-resolved real-time fluorescent quantitative PCR system developed by a skilled international technical team. Featuring a novel optical path system utilizing Fresnel lenses, time-resolved signal acquisition technology, and unique temperature control innovations, QuantaVue offers enhanced detection sensitivity, improved temperature control precision and uniformity, simplified operation, and expanded analytical capabilities. Moreover, adhering to a global product design and manufacturing perspective, QuantaVue delivers an exceptional international standard of quality.

Description

Innovative Optical Design for Higher Sensitivity: The Unicorn system utilizes a patented new optical detection system with Fresnel lens and large area MPPC, enhancing detection sensitivity. It minimizes optical cross-talk between samples by bringing the detector closer to the sample, ensuring better sensitivity. Time-resolved well-by-well scanning technology reduces cross-talk, and high-frequency LED/MPPC pairing minimizes fluorescence quenching.

Outstanding Thermal Cycler: The Unicorn system boasts superior uniformity, precision, and speed. It employs Peltier components, a hollow-out thermal block for rapid temperature changes, temperature compensation technology, and precise temperature control. It maintains a tight temperature uniformity of ±0.2°C and achieves heating rates of up to 6°C/s. It’s compatible with various sample formats, including 96-well plates, 8-strip tubes, and single tubes.

Impressive Performance for High-Quality Data: The Unicorn system guarantees ideal amplification results with its innovative optics and precise temperature control. It offers excellent reproducibility, a wide dynamic range, and precise quantification, making it a reliable choice for quantitative real-time PCR applications

Special Analyzer Software: The Unicorn Analyzer Software is user-friendly and highly customizable. It offers intuitive navigation and settings, integrated analysis algorithms with automatic analysis, and comprehensive analysis modules for multiple applications. It’s license-free and provides free updates. The software simplifies experiment setup, auto-analysis, and personalized data export.

Comprehensive Functionality: The software includes built-in data analysis modules with automatic baseline subtraction, threshold calculation, and standard curve generation. It supports absolute and relative quantifications, probe-based allelic discrimination, and positive/negative analysis. Melt curve analysis, protein stability screening, and various analysis methods are also available, ensuring a wide range of applications.

Specifications

ModelQuantaVue 4X
Optical detection
Excitation light source4 monochrome high efficiency LED(maintenance-free,>100000h)
Fluorescence channel number4-channel
Supporting dyeF1: FAM/SYBR Green etc.
F2: Cy5 etc.   F3: VIC/HEX/JOE /TETetc.
F4: FAM/SYBR Green etc.  F5: ROX/Texas Red etc.
Detection deviceHighly sensitive silicon photomultiplier(MPPC)Detection modeTime resolved real-time scanning
Fluorescence reading positionTop readExcitation/detection range455-680nm/510-730nm
SensitivitySingle copy geneResolutionIn the single reaction, 1.33 times copy number difference can be distinguished
Dynamic rangeTen orders of magnitude(1-10^10 copy)
Temperature control
Sample capacity96Thermal cycling techniquePeltier(Six temperature control modules)
Reaction volume10-50μlMaximum module rise and cooling rate6℃/s
Temperature range4-100 ℃Temperature accuracy≤±0.2℃
Hot cover techniqueElectronic automatic hot cover(Adjustable from room temperature to 115℃)Temperature uniformity≤±0.2℃
Number of temperature gradient12 columnsTemperature gradient range of temperature difference1-40 ℃
Analysis mode
Absolute quantitative、relative quantitative、qualitative analysis、end-point fluorescence analysis、melting curve、high resolution melting curve(HRM)、genotyping
Data export
It can be exported in CSV, Excel, txt and other formats. The user report contains experimental attributes, operation Settings, data results, original data and other information.
Graphic and tabular data results can be printed directly or saved in PDF format;
Experiment report format can be customized;
Prestore a variety of experimental report modules.
Product qualification
NMPA Class III Medical Device Registration Certificate、CE-IVD authentication

Brouchure

Real-time PCR QuantaVue 4X - Unicorn Medical 2023.pdf (1220 downloads )

Download the brochure

Uncover the cutting-edge QuantaVue 4X series, the culmination of Unicorn’s quantitative PCR technology. Designed by a talented international technical team, this time-resolved real-time fluorescent quantitative PCR system showcases innovative features such as a unique optical path system with Fresnel lenses, time-resolved signal acquisition technology, and temperature control breakthroughs. Experience heightened detection sensitivity, precise temperature control, and a streamlined operation. Our brochure delves into the QuantaVue 4X series, ensuring you’re at the forefront of quantitative PCR technology. Download it now and elevate your research.

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