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Class II Type B2 (Total Exhaust) Biological Safety Cabinet

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The Class II biological safety cabinet is a negative pressure safety device which is mainly used for handling pathogenic biological samples or for applications that require a sterile work zone. It adopts advanced air purification technology and negative pressure box design, and it realizes the protection of the environment, users and samples.

A biological safety cabinet is designed to have: an inflow for protecting the operator from harmful agents inside the cabinet, an ULPA filtered downflow which creates an ISO Class 3 work surface that avoid contamination of the work, experiment or process, and an ULPA filtered exhaust for containing contaminants to protect the environment. It is the most basic safety equipment in laboratory biosafety protection.

Class II Type B2 Biosafety Cabinet is designed to provide operator, product, and environmental protection in experiments where recirculation of filtered air is prohibited, most likely due to the presence of chemical fumes emitted during the process.

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Description

ULPA Filtration System

  • 10 x filtration efficiency compared to HEPA filter, creating ISO Class 3 cleanliness instead of industry-standard ISO Class 5
  • Utilize ULPA filter (per IEST-RP-CC001.3)/H14 per EN1822 instead of H13 HEPA filters used on many BSCs in the market
  • 99.999% at 0.1 to 0.3 micron, ULPA as per IEST-RP-CC001.3 USA or >99.999% at MPPS, H14 as per EN 1822 EU
  • Leak-free performance test is carried out regarding structural stability and filter integrity prior to product delivery

High Performance Blower

  • Stable airflow speed despite voltage fluctuations and filter loading by automatic air volume compensation.
  • Communicates with airflow sensor through microprocessor, there is no need for additional manual speed control
  • Compact and energy-efficient motor runs cooler than other comparable motors, and consumes less energy.
  • Quite operation for any comfortable lab conditions. Longer average motor life eliminates service cost.

Airflow Velocity Monitoring

  • Imported velocity meter set a new standard in precise downflow airflow speed monitoring to ensure a safe operation.
  • Velocity sensor provides instant feedback to the central control system. Auto-adjust BSC airflow as filters load to keep you safe.
  • Continuously monitors and maintains inflow and downflow at real-time, and indicated on the large LCD display.
  • Alarm thresholds (±10% of the original set point) are strictly controlled. Alerts will be activated if airflow is insufficient.
  • High quality pressure sensor indicate real-time negative pressure between two sides of the downflow filter.

Touch Screen Control Panel

  • Easy reach & viewing all safety information and performance data on a large touchable screen from a seated position.
  • Intuitive interface with easy-to-understand graphics displays airflow velocities, blower condition, filter life, sash status, alarm messages.
  • Engage the security lock feature to prevent access to the cabinet by unauthorized or unfamiliar users
  • Diagnostics button to easily check the cabinet operating parameters and assist servicing.

Robust Construction

  • All biological safety cabinet structures are subjected to pressure decay method to prevent leakage.
  • Seamless, non-porous, autoclavable SUS304 for working table, one-piece side/rear walls and bottom sink.
  • Large radius corners is easily cleanable. No welded joints in the work area, reducing the growth of bacteria.
  • Removable and divided table top is also available, which is easy to lift, thorough clean and further sterilization.

Illumination & UV sterilizing

  • High quality and brightly fluorescence lamp provides sufficient and uniform illumination for maximum visibility.
  • Built-in hidden light tubes ensure excellent protection the eyes of operator from harm and also reduce operator fatigue.
  • Interlocking function is integrated in the cabinet’s control system for UV lamp, fluorescent lamp, front sash window.
  • Powerful UV irradiation ensures thorough disinfection. UV lamp appointment saves sterilization waiting time.

Tempered Glass Door

  • Front sash designed with unobstructed by exterior mounted sash handles ensures convenient use and precise sash opening height position.
  • 10°backward-slanted safety grade glass provides a close and comfortable view, with a protection from explosion and UV.
  • Counterbalanced sliding sash supported by unique manual lifting system can be raised to a maximum height for easy introduction of large items.

Support Stand

  • Support stand is provided to ensure the cabinet stability and ergonomic working position.
  • Detachable frame stand is convenient for installation, saving valuable lab space and shipping space as well.
  • Manual adjustable leveling feet to compensate for uneven floor. Available with castors for easy re-location or transportation.

Recepticles & Service Fixtures

  • Waterproof and overload protection. Electrical outlet cover can be opened with one finger and then gently and slowly self-closes.
  • Easy to reach service fixture, access for various valves for choice, including Gas, Vacuum, Air, Nitrogen, Water, Universal and etc.
  • Optional Vacu-Pass Cord & Cable portal kit for passage of a cable through right-side wall. accommodates cords/cables with different diameters.

Specifications

ModelBSC-1000IIB2BSC-1300IIB2BSC-1600IIB2
Type
Type of BSCClass Ⅱ Type B2Class Ⅱ Type B2Class Ⅱ Type B2
DimensionsNominal Size3 feet4 feet5 feet
Usable Working Area0.672㎡
7.23 sq.ft.		0.8736 ㎡
9.40 sq.ft.		1.0752 ㎡
11.57 sq.ft.
Stand Height (mm)640mm（Adjustable）640mm（Adjustable）640mm（Adjustable）
Internal Work Area,
Dimensions(WxDxH)		1000*672*630mm1300*672*630mm1600*672*630mm
External Dimensions
with Base Stand(WxDxH)		1180*790*2080mm1480*790*2080mm1780*790*2080mm
AirflowAirflow Mode100% exhaust100% exhaust100% exhaust
Exhaust DirectionTop ExhaustTop ExhaustTop Exhaust
Average Inflow Velocity0.53m/s0.53m/s0.53m/s
Average Downflow Velocity0.35m/s0.35m/s0.35m/s
Inflow Airflow Volume1141m3/h1484m3/h 1826m3/h
Downflow Airflow Volume798m3/h1038m3/h1277m3/h
Exhaust Airflow Volume1141m3/h1484m3/h1826m3/h
Velocity Meter Accuracy0.001m/s0.001m/s0.001m/s
ConstructionBlowerACACAC
Downflow filterULPAULPAULPA
Exhaust filterULPAULPAULPA
Main Body1.2mm (xxx'') 18 gauge Electro-galvanized steel with white oven-baked powder-coated finish
Work Zone1.5mm (xxx'') 16 gauge Stainless steel, type304, Single-piece or 3-piece
Side Walls1.5mm (xxx'') 16 gauge Stainless steel, type304
Sash Glass Thickness5mm5mm5mm
Sash Glass TypeTempered Glass, UV-proofTempered Glass, UV-proofTempered Glass, UV-proof
Sash Window Working Opening180mm180mm180mm
Sash Tested Opening180mm 180mm 180mm
Sash Maximum Opening530mm530mm530mm
Fluorescent Light Intensity≥700lux ≥700lux ≥700lux
RMS≤3um≤3um≤3um
Sound Emission (dBA)≦65dBA≦65dBA≦65dBA
CleanlinessCleanlinessULPA: Class 3(ISO 14644.1); Class 100 (Fed 209E)
Filter Typical Efficiency＞99.999% at 0.1 to 0.3 micron, ULPA as per IEST-RP-CC001.3 USA
Total colony in impaction sampler (Operator Protection)≤10CFU./Time≤10CFU./Time≤10CFU./Time
Total colony in slit type sampler (Operator Protection)≤5CFU./Time≤5CFU./Time≤5CFU./Time
Total colony in culture dish (Prooduct Protection)≤5CFU./Time≤5CFU./Time≤5CFU./Time
Total colony in culture dish (Cross contamination)≤2CFU./Time≤2CFU./Time≤2CFU./Time
Personnel Protection TestKI-Discus containment and microbiological testing are performed
ControllerDisplay7 inches touch screen7 inches touch screen7 inches touch screen
UV TimerYesYesYes
Main Power SwitchYesYesYes
Electrical DataCabinet Full Load Amps (FLA)2.2A2.2A2.2A
Optional Outlet (FLA)6A6A6A
Norminal Power Consumtion (W)1500W(Excluding external exhaust fan)1500W(Excluding external exhaust fan)1500W(Excluding external exhaust fan)
Power SupplyAC220±10%,50/60HZAC220±10%,50/60HZAC220±10%,50/60HZ
AlarmAlarm TypeAudible + VisualAudible + VisualAudible + Visual
Inflow VelocityYesYesYes
Downflow VelocityYesYesYes
Sash Height limitYesYesYes
Filter BlockedYesYesYes
Filter Lifetime RemindYesYesYes
AccessoriesFluorescent lamp30W*230W*230W*2
Ultraviolet lamp30W*130W*130W*1
Receptacle (Pieces/Power/Current)111
Detachable Base StandYesYesYes
WheelsYesYesYes
Gas ValveOptionalOptionalOptional
Vcuum ValveOptionalOptionalOptional
Water ValveOptionalOptionalOptional
Arm RestOptionalOptionalOptional
I.V. PoleOptionalOptionalOptional
Canopy connectionOptionalOptionalOptional
WeightNet Weight (kg)280 Kg (617 lbs)300Kg (661 lbs)330 Kg (727 lbs)
Gross Weight (kg)305Kg (606 lbs)330Kg (661 lbs)370 Kg (750 lbs)
ShippingPacking size (WxDxH)1310*950*2220mm1610*950*2220mm1910*950*2220mm
Shipping Volume3.06m³(108.06 ft3)（Including external exhaust fan）3.70 m³(130.66 ft3)（Including external exhaust fan）4.33 m³(152.91 ft3)（Including external exhaust fan）
StandardCertificatesCE, EN12469, ISO9001, ISO13485, CFDA YY0569

Accessories

ModelBSC-1000IIA2BSC-1300IIA2BSC-1600IIA2
Fluorescent lamp30W*230W*230W*2
Ultraviolet lamp30W*130W*130W*1
Receptacle111
Detachable Base StandYesYesYes
Gas ValveOptionalOptionalOptional
Vcuum ValveOptionalOptionalOptional
Water ValveOptionalOptionalOptional
Arm RestOptionalOptionalOptional
I.V. PoleOptionalOptionalOptional
Canopy connectionOptionalOptionalOptional

Brouchure

Clean Air Solution English Brochure V2023

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Gold standard of manufacturing

From a heritage of more than decades of experience, Unicorn Medical has contribute to the development of life science, clinical, pharmaceutical, industrial, and food industry by innovative solutions for air containment, contamination control and controlled environments. We realize how important to offer a high level of protection to operator, sample and environment with our unique and advanced technologies. With an extensive track record of reliability, safety and performance, We provide higher quality products to meet different applications. Unicorn operates under ISO9001, ISO14001 and ISO13485.

Unicorn air containment and protection product line includes biological safety cabinets, laminar flow cabinets (clean benches), clean booth, dispensing booth, air purifier, air shower, pass box, fan filter units, clear partitions, pressure dampers, etc.

From our headquarters in China, Unicorn initiates an efficient research, product development, manufacturing and dedicated customer service program. As you learn more about Unicorn, you will understand why we deliver innovative laboratory equipment solutions worldwidely.

BSC factory looking 300-300
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BSC workshop 300-300
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Your reliable life science partner

Our Difference

Unicorn Biological Safety Cabinet is an effective solution in providing operator, product and environmental protection. It also features stable and self-compensating airflow, despite building voltage fluctuations & filter loading.

Unicorn biological safety cabinets exceed current conventional BSC standards and offer outstanding safety when things don’t go as expected. Unicorn gives you MORE.

Multiple technology, Outstanding comfort, Reliable Safety and Energy efficiency you can rely on. That’s the Unicorn difference.

Technology

Quite, energy-saving motor works with intelligent control system, keeping precise airflow even during ULPA filters loading or temporary obstruction.

Safety

Each cabinet is designed and produced with a variety of core components and features to improve cleanliness and eliminate sample contamination risk.

Comfort

Large colored touch screen for easy monitoring work status, angled sash with more convenient viewing , and adjustable telescope type stands available.

Efficiency

Blower save on energy while providing longevity and comfort. The fluorescence light and UV with appointment function consume less. Over 70% recycled materials.

Compliance With Global Standard

Biosafety cabinetsEN 12469, Europe; SFDA YY0569, China
Air QualityISO 14644.1, Class 3, Worldwide
US Fed Std 209E, Class 100, USA
FiltrationISO 29463, Worldwide
EN 1822, Europe
EN 13091, Europe
IEST-PR-CC001.3, Worldwide
IEST-PR-CC007.1, Worldwide
IEST-PR-CC034.1, Worldwide
Electrical SafetyEN 61010-1, Europe
IEC 61010-1, Worldwide
EN 61326-1, Europe
IEC 61326-1, Worldwide
Quality ManagementISO9001:2008, ISO13485:2003
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Airflow Pattern

Class II Biological Safety Cabinet

Ambient air is pulled through front grille to create inflow, without going into the work surface. Inflow is joined by half of the downflow to create front air curtain that is fine-tuned to create a large performance envelope. The combined air stream travels through the back air column towards the blower.

Approximately 1/3 of the air in the common plenum is exhausted through the ULPA filter to the room. The remaining 2/3 of the air is passed through the downflow ULPA filter and into the work area as a vertical laminar flow air to creat ISO Class 3 work surface and prevents cross contamination

Near the work surface, the downflow splits. About half goes to the front grille. And half goes to the rear grille. A small portion enters the side capture zones to prevent dead corners

The design was optimized to give large performance envelope, that provides operator and product protection at wide inflow and downflow variation form the nominal point.

With a range of biological safety cabinet, Unicorn has the right technology to meet different application, setting, and budget.

Class II Type A2 VS Class II Type B2 Biosafety Cabinet

Biological Safety Cabinet is a primary engineering control which provides user protection against biohazard as the inflow air creates airflow barrier preventing accidental release of biohazards from the cabinet’s working area and at the same time provides product protection with the airflow barrier inside the work zone which is on the other hand created by the downflow air.

ClassificationClass II Type A2Class II Type B2
ProtectionProduct, Personnel, EnvironmentProduct, Personnel, Environment
Biosafety Level*1,2,3 and 41,2,3 and 4
DuctingRecirculate to room or vented with Canopy connection & ductwork100% Vented (Total Exhaust) **
Energy Requirement$$$$
Design work-+
Flexibility+++
Typical ApplicationCell/Tissue culture
Microbiology
Pathology/Clinical
Pharmacy
Work involving minute volumes of chemicals***		All A2 applications, Plus: Work with larger volumes of volatile chemicals
BiotechnologyMedium Preparation
Tissue Culture
Blood Elements Analysis
Human Histology
Polymerase Chain Reaction		Medium Preparation
Tissue Culture
Blood Elements Analysis
Human Histology
Polymerase Chain Reaction
MicrobiologyMedium Preparation
Isolated clinical sample
Blood test/analysis
QA/QC
Minute Quantities of Volatile Toxic Chemicals
Trace Amount of Radionucleotides		Medium Preparation
Culture nuisance odors
Isolated clinical sample
Blood test/analysis
QA/QC
Minute Quantities of Volatile Toxic Chemicals
Trace Amount of Radionucleotides
PharmaceuticalGeneral applicationsAntitumor drug preparation
Trace Amount of Radionucleotides
Routine researchToxic Powder/ Suspended SubstanceCell/Tissue Immobilization & Staining
Toxic Powder/ Suspended Substance

*Biosafety in Microbiological and Biomedical Laboratories (BMBL) 6th Edition.

**Dedicated ductwork required for each Type B2 cabinet per CETA CAG-007

***Canopy connection and exhaust ductwork required.

Service Access and Validation

Thanks to front open design, all services, adjustments and filter change can easily accessible from the front of the cabinet, allowing for rapid service and minimal work disruption.

All service is perfomed with accessibility and cleanability to prevent unnecessary contamination. 

Unicorn offers a wide range of high quality services for all our biological safety cabinets.

Service

All service is performed with accessibility and cleanability to prevent unnecessary contamination, including:

  • Fluorescent lamps
  • UV lamps
  • Change of HEPA filter
  • Adjustable or change of circuit boards and sensors
  • Adjustment to alarms, fan speed, and control panel

 

Validation

Unicorn offers professional validation services for all our equipment, including:

  • Installation qualification
  • Operational qualification
  • Calibration
  • IQ/OQ Protocols are available

Comprehensive Performance Testing

Additional KI-Discus containment and microbiological testing are performed on statistical sampling basis.

Each Biosafety Cabinet produced by Unicorn is individually tested, documented by traceable serial number and validated with the following ways:

  • Inflow/Downflow velocity testing
  • PAO aerosil challenge for filter integrity
  • Airflow pattern visualization
  • UV radiation test
  • Illumination test
  • Vibration test
  • Air particle count test
  • Microbiological test (bacillus subtilis)
  • KI-Discus containment test
  • Electrical safety to IEC-61010 and IEC 61326

Download the brochure

If you would like to  know more about Unicorn Medical’s air containment and protection product line includes biological safety cabinets, laminar flow cabinets (clean benches), clean booth, dispensing booth, air purifier, air shower, pass box, fan filter units, clear partitions, pressure dampers, etc.

Please submit the following form and get the latest brochure from Unicorn. 

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