AccuONE-200 Digital PCR

The digital PCR system can evenly divide the PCR reaction system into tens of thousands of reaction droplets, each droplet completes PCR amplification in parallel and independently, and makes statistics by judging the presence or absence of fluorescent signals after the amplification of each droplet is completed, and finally according to The Poisson distribution formula is used to calculate the initial concentration or copy number of the target gene in the original system. The digital PCR system can be applied in: tumor liquid biopsy, non-invasive prenatal diagnosis, pathogenic microorganism detection, viral load determination, copy number variation, rare mutation detection, relative gene expression research, transgenic detection, next-generation sequencing result verification and other fields.

  • Chip utilization rate is 100%, independently
  • Test is completed within 2 hours
  • 6 fluorescence channel, probe/dye
  • Quadruple chip (15K/20K/28K) & Quintuple chip (15k)
Category:

Description

Technical Advantages

  • Micro-cavity chip: MEMS processing, micro-nano chip processing, uniform chamber volume
  • Automated preparation: One-key preparation of microsystems, no need for manual pipetting, avoiding errors caused by different personnel operations.
  • 6 color fluorescence: The biochip reader is equipped with a 6-color fluorescence detection system, which can realize 6 multiplex detection in a single sample, saving samples and significantly improving detection efficiency.
  • Various options:  Chips with four unit numbers of 15k, 20k, 28k  & five unit number of 15k are provided. probe method, dye method, and one-step method dPCR Master Mix.
  • Open system: Compatible with a variety of mainstream brand reagents (Thermo, Roche, Qiagen, Takara, Transgene, Yeasen, etc.), users only need to make simple adjustment to the above reagent systems to quickly achieve compatibility
  • Direct: Interpretation by endpoint method, no standard curve required.
  • Sensitive: Single copy detection, suitable for low concentration samples
  • Stable: Not susceptible to inhibitory factors & amplification efficiency

Application

  • Molecular biology: Gene expression, CNV, Methylation
  • Medical research: Liquid biopsy, NIPT, Tumor, Genetic disease
  • Pathogens: HIV, HBV, HCV, nCoV, Bacteria, Fungus, Mycoplasma, Chlamydia
  • Bio-pharma: Process QC, Drug evaluation
  • Agriculture/Food: GMO, Genetic breeding, Food safety
  • Environmental: Soil, Water

Workflow

  1. System preparation: Mix nucleic acid template, digital PCR Master Mix, primers, probes and other PCR components in proportion
  2. Micro-unit generation: The sample preparation instrument completes the generation of tens of thousands of micro-units
  3. PCR thermal cycler: Complete gene amplification on the gene chip amplification instrument
  4. Chip reader & analysis: Chip photography and imaging, software analysis and calculation of target concentration.

Digital PCR Master Mix

  • DigitalAmp PCR Master Mix (10x):  Suitable for high sensitivity ultra-multiplex dPCR amplification reaction
  • One-step RT-dPCR Mix (5x): One-step reaction system is applied, and RNA is directly amplified.
  • DigitalAmp PCR Master Mix (SYBR Green): Suitable for dye method, efficient and stable

 

Specifications

ModelAccuONE-200
Micro-reaction unitmicro-cavity chip, solid phase segmentation
Bio-chip type10k, 22k, 120k option, other type is customizable
Dropelet preparationPipetting without additional micro-droplet generation system
Reaction volume15ul standard, adjustable within the 40ul range
Sample preparation time≤10 seconds/piece
Excitation light sourceHigh-efficiency maintenance-free LED light source
DetectorHigh-resolution CMOS sensor
Valid time of chip readingRead repeatedly within 2 weeks
Number of fluorescence channel6
Compatible dyesAtto425, FAM, SYBR Green, EvaGreen, VIC/HEX, JOE, CY3, TAMARA, ABY, ROX, JUN, TYE655, CY5, Texas Red, CY5.5 and other similar wavelength dyes
Sample detection throughputat least 12 samples at one time
Daily detection throughput360 in a single day (8 hours);
Sample detection time≤1 minute/piece, ≤12 minutes/12 pieces
Detection sensitivity≤0.001%, can detect single-copy genes
Dynamic range≥5 orders of magnitude, 1~250000 copies/sample
Reagent versatilityCompatible with probe method and dye method
Supporting reagents10× high-concentration DNA detection reagent and 5× RT-dPCR one-step RNA detection reagent
Maximum sample input≥12 µl
SoftwareCalculation of copy number, copy number concentration, mutation abundance, confidence interval range, accuracy; threshold line automatic or manual division, single or unified threshold division; output excel data, two-dimensional scatter plot, two-dimensional bar chart, three-dimensional space map; automatically identify complex droplet clusters, output chip actual hole position discrimination map; data quality control function, etc. automatically generate test reports;
Data securityPermission management, auditing and electronic signature functions to ensure the validity and reliability of data and meet FDA 21 CFR Part11 compliance requirements
Power supply220V/50Hz-60Hz
Dimension (W×D×H, mm)Thermal cycler: 480mm×330mcm×340mm， Biochip reader: 475×385×308mm
Net weight (KGS)Thermal cycler: 8.9 ，Biochip reader: 12

Ordering Information

Digital PCR Instrument

NameNoteOrder No.Format
Gene amplifierMicro-unit amplificationIN02021PC
Biochip reader (6 colors)Bio-chip readingIN03101PC

Digital PCR Reagent & Consumable

NameNoteOrder No.Format
dPCR biochip boxBiochip version 2.0CM020432T/box
Seal oilChip oil sealCM0102100T
10× probe method mix (including UDG)Applied to probe methodMX0109100T
10× Eva dye mix (including UDG)Applied to dye methodMX0110100T
10× dPCR Taq Master Mix(ROX, UDG-free)Applied to probe methodMX0111100T
One-step mix for bio chips, ROX substrateApplied to probe methodMX0203100T

Brouchure

AccuONE-200 Digital PCR System - Unicorn Medical 2024 (33 downloads )

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