Description
Technical Advantages
- Fast: Single-chip reading can be completed in 5 seconds
- Flexible: There is no need to wait for enough samples, each part of the instrument can be expanded, upgraded, and used independently
- Compact: The machine is designed withe footprint of only the size of a palm, saving valuable space
- Open: Compatible with a variety of mainstream brand reagents (Thermo/ Roche/ Qiagen/ Takara/ Transgene, etc.)
- Direct: Interpretation by endpoint method, no standard curve required.
- Sensitive: Single copy detection, suitable for low concentration samples
- Stable: Not susceptible to inhibitory factors & amplification efficiency
Application
- Molecular biology: Gene expression, CNV, Methylation
- Medical research: Liquid biopsy, NIPT, Tumor, Genetic disease
- Pathogens: HIV, HBV, HCV, nCoV, Bacteria, Fungus, Mycoplasma, Chlamydia
- Bio-pharma: Process QC, Drug evaluation
- Agriculture/Food: GMO, Genetic breeding, Food safety
- Environmental: Soil, Water
Workflow
- System preparation: Mix nucleic acid template, digital PCR Master Mix, primers, probes and other PCR components in proportion
- Micro-unit generation: The sample preparation instrument completes the generation of tens of thousands of micro-units
- PCR thermal cycler: Complete gene amplification on the gene chip amplification instrument
- Chip reader & analysis: Chip photography and imaging, software analysis and calculation of target concentration.
Digital PCR Master Mix
- DigitalAmp PCR Master Mix (10x): Suitable for high sensitivity ultra-multiplex dPCR amplification reaction
- DigitalAmp PCR Master Mix (SYBR Green): Suitable for dye method, efficient and stable
- One-step RT-dPCR Mix (5x): One-step reaction system is applied, and RNA is directly amplified.