Transforming Cancer Care: Mandaya Royal Hospital Puri’s Journey with Unicorn Digital PCR
November 1, 2023
No Comments
Read More »
Protect human health and help life exploration
Flagship model, dual-module integrated digital PCR system, 6-color fluorescence channels, and a maximum throughput of 48.
6-color fluorescence, detection throughput 1 to 16, suitable for a variety of specifications of chips, the best and powerful performance.
1~2 color fluorescence (FAM/VIC), extremely fast reading, the world’s smallest, most flexible, and most economical digital PCR. This model is an excellent choice for popular use, for most customers do not pursue high throughput and ultra-multiple detection capabilities.
CMOS imaging, 6 color detection channel enables multiplex PCR testing of 6 target genes to be determined simultaneously in a single sample, reducing the detection cost per unit point.
Stable and efficient thermal cycler is provided. with high performance and accuracy. The daily detection throughput can be upgraded by increasing the number of gene amplifiers.
Fully automatic and pollution-free sampling technology eliminates differences in the operation of different personnel. High sample utilization and high number of effective droplets
With intuitive user interfaces and powerful algorithms, you can confidently extract valuable insights from your digital PCR experiments. unlocking full potential of dPCR experiments.
Low cost and customized biochip options (10K-50K/ 10ul-30ul). MEMS processing, the etching accuracy of the lithography machine can reach the nm level. and the micro-cavity is uniform
Specific, high amplification efficiency, with different options, including probe method, dye method, one-step RT-dPCR mix, supporting open reagent development
Our state-of-the-art biochips represent the pinnacle of digital PCR technology, offering unparalleled precision and reliability for your most demanding molecular applications.
Key Features:
Benefits:
Introduce PCR solution and sealing oil sequentially into the chip, with the reaction mixture preceding the oil phase for optimal distribution and containment.
Allow PCR solution to flow into microcavities, forming discrete droplets, while sealing oil simultaneously creates a protective barrier at the chip’s bottom surface.
Complete droplet generation process as oil fully seals the lower chip boundary, ensuring isolation of individual reaction compartments for precise analysis.
Administer additional oil to the upper chip surface, effectively sealing all compartments and finalizing the chip preparation for subsequent PCR amplification.
High precision, high sensitivity
Common specifications
Cost effective
Suitable for probe method, suitable for low nucleic acid copy sample detection
Suitable for dye method, efficient and stable.
A one-step reaction system was applied, and RNA was directly amplified.
Our state-of-the-art software features designed specifically for digital PCR. We understand the critical importance of accurate and efficient data analysis in the field of digital PCR, and that’s why we’ve developed a comprehensive suite of software tools to streamline your workflow. Our software enables precise quantification of target molecules, effortless data visualization, and advanced analysis capabilities. With intuitive user interfaces and powerful algorithms, you can confidently extract valuable insights from your digital PCR experiments. Whether you’re performing gene expression analysis, mutation detection, or rare event quantification, our software is tailored to meet your specific needs. Experience the convenience and reliability of our software features, and unlock the full potential of your digital PCR experiments.
The strategy of digital PCR – “divide and rule”. A standard PCR reaction is allocated to a large number of micro reactors, and each reactor contains or does not contain one or more copies of the target molecule (DNA template) to achieve “single molecule template PCR amplification”. After amplification, the number of positive wells is “counted” by the number of positive reactors, and then the number of positive copies is calculated according to Poisson’s formula.
| Comparison | qPCR | dPCR |
|---|---|---|
| Principle | The ct value was determined according to the real-time fluorescence curve | The copy number of the target sequence was calculated statistically according to the signals of reaction units |
| Quantitative type | indirect | direct |
| Sensitivity | high | Extremely high |
| Accuracy | Poor repeatability at low concentration | good repeatability |
| Tolerance of inhibitory factors | Weak, greatly affected by amplification efficiency | Strong, less affected by inhibitory factors |
| Application | gene expression level pathogen detection genotyping … | viral load CNV gene mutation rate … |
Digital PCR (dPCR) revolutionizes the field of molecular biology by offering precise and sensitive detection of nucleic acids. Its applications span a wide range of disciplines, driving advancements in various research areas. From cancer research and infectious disease diagnostics to environmental monitoring and food safety analysis, dPCR provides a powerful tool for quantifying rare targets, detecting genetic mutations, and analyzing gene expression with unparalleled accuracy. With its ability to precisely measure nucleic acid concentrations, detect low-abundance targets, and provide absolute quantification, dPCR opens doors to new possibilities in precision medicine, personalized genomics, and biomarker discovery. Whether you’re investigating tumor heterogeneity, studying pathogen dynamics, or exploring genetic variations, dPCR empowers researchers with unprecedented insights and the potential to unlock groundbreaking discoveries.
Discover how digital PCR revolutionizes cancer diagnostics with its unparalleled sensitivity and precision. This video showcases the applications of digital PCR in detecting minimal residual disease (MRD) in cancer patients, providing early insights for efficient treatment monitoring and prognosis assessment. Learn how this advanced technology is reshaping personalized medicine and improving patient outcomes.
Welcome to the cutting-edge world of digital PCR technology! Our state-of-the-art digital PCR solutions are transforming industries by offering unparalleled precision and reliability. From groundbreaking research in biotechnology to critical applications in clinical diagnostics, environmental monitoring, and food safety testing, our technology is at the forefront of innovation. Here, you’ll discover inspiring stories of how our clients across diverse sectors are harnessing the power of digital PCR to achieve remarkable outcomes. Dive into these cases to see the real-world impact of our technology and envision how it can elevate your work to new heights. Explore, get inspired, and let’s redefine the possibilities together!
Unicorn Medical Molecular Business product line was established in 2016. We are committed to the R&D, production and sales of instrument consumables in the field of life science and technology. The core team includes not only business management experts and marketing experts from world-renowned medical device companies, but also micro-nano processing, precision instruments, image algorithms, biological reagents and applications. scientists and technologists. Our company is in a stage of rapid development. We sincerely invite outstanding talents to join us and create great achievements together!
If you would like to know more about Unicorn Lifescience’s Digital PCR product line including AccuMini dPCR, AccuMini Plus dPCR, AccuONE-200, AccuONE-300 Digital PCR systems.
Please submit the following form and get the latest brochure from Unicorn.
Deliver the best with innovative life science solutions