Microvolume-Spectrophotometer.png

Microvolume Spectrophotometer

The fluorometer is combined with a quantitative test kit, which uses fluorescent dyes to selectively bind to specific target molecules. These fluorescent dyes can only emit fluorescent signals when they bind to target molecules.
Therefore, it is more accurate than the traditional UV absorption method, because the UV absorption method is not selective, and measures the absorbance value of all substances at 260—such as DNA, RNA, protein, degraded nucleic acid and free nucleotides or excess salt ions, etc. In addition, the high-sensitivity fluorometer is specially used to detect the concentration range lower than the detection limit of the trace ultraviolet spectrophotometer. The FluoONE fluorometer combined with the corresponding quantitative kit can quickly, sensitively and accurately determine the concentration of DNA.

Category:

Description

Fast detection
There is no need to dilute during the detection process, the trace sample is added directly, no other consumables are needed, and the detection can be completed in about 6 seconds.

Long-life light source, no need to warm up when starting up
Xenon flash lamp with a lifespan of up to 1 billion times (up to 10 years). There is no need to warm up when starting up, and it can be detected directly.

High concentration detection range
The highest detectable sample concentration is 15000ng/μL, and the sample does not need to be diluted.

Easy to use
Drop the sample directly on the sample stage without dilution or other consumables, and the measurable sample concentration is 300 times than conventional spectrophotometer
Detection arm closure can be automatically detected, simplifying operation

Fluorometer mode
For samples with a concentration lower than 2ng/uL, the fluorometer mode can be selected, and the lowest detection limit can reach 0.5pg/uL.
Compatible with common commercial fluorescent quantitative reagents, providing users with the greatest convenience and the lowest detection cost

Various models
Nano-1000/Nano-1000F is a full-wavelength micro-spectrophotometer, and Nano-2000/Nano-2000F is a fixed-wavelength spectrophotometer

Specifications

ModelNano-1000Nano-1000FNano-2000Nano-2000F
Wavelength range190~850nm190~850nm260nm,280nm,365nm260nm,280nm,365nm
Sample volume0.5~2ul0.5~2ul0.5~2ul0.5~2ul
Path length0.05mm, 0.2mm, 1.0mm, auto-ranging0.05mm, 0.2mm, 1.0mm, auto-ranging0.2mm, 1.0mm0.2mm, 1.0mm
Light source/Life spanXenon Flash/10^9 timesXenon Flash/10^9 timesUV LED/8000hUV LED/8000h
Detector type2048-element Linear CCD Array2048-element Linear CCD ArrayUltraviolet silicon photocellUltraviolet silicon photocell
Spectral bandwidth2nm2nm8nm8nm
Display7 inch touch screen7 inch touch screen7 inch touch screen7 inch touch screen
Absorbance precision0.003Abs0.003Abs0.005Abs0.005Abs
Absorbance accuracy±1%(7.332Abs at 260nm)±1%(7.332Abs at 260nm)±5ng or 2%±5ng or 2%
Absorbance range0.04-300A (10mm equivalent)0.04-300A (10mm equivalent)0.2-100 (10mm equivalent)0.2-100 (10mm equivalent)
Nucleic acid range2ng/ul~15000ng/ul dsDNA2ng/ul~15000ng/ul dsDNA5ng/ul~5000ng/ul dsDNA5ng/ul~5000ng/ul dsDNA
Measurement time6 sec6 sec6 sec6 sec
Auto-calibrationYesYesYesYes
OD600 Absor. range0~4.000 Abs0~4.000 Abs0~4.000 Abs0~4.000 Abs
OD600 Absor. stability[0,3) ≤0.5%, [3,4) ≤1%[0,3) ≤0.5%, [3,4) ≤1%[0,3) ≤0.5%, [3,4) ≤1%[0,3) ≤0.5%, [3,4) ≤1%
OD600 Absor. Repeatability[0,3) ≤0.5%, [3,4) ≤1%[0,3) ≤0.5%, [3,4) ≤1%[0,3) ≤0.5%, [3,4) ≤1%[0,3) ≤0.5%, [3,4) ≤1%
OD600 Absor. accuracy[0,3) ≤0.005A+1%, [3,4) ≤2%[0,3) ≤0.005A+1%, [3,4) ≤2%[0,3) ≤0.005A+1%, [3,4) ≤2%[0,3) ≤0.005A+1%, [3,4) ≤2%
Fluorescence measurement/Excitation wavelength:470nm;Emission wavelength:525nm/Excitation wavelength:470nm; Emission wavelength:525nm
Fluorescence linearity/R2>0.995/R2>0.995

Brouchure

Certificates

Related products